F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.^[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.^[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.^[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.^[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.^[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.^[7]

F-CHP is labeled with fluorescein for direct fluorescence detection.

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).^[3,7]

Applications: immunofluorescence,^[3] cell imaging,^[4] SDS-PAGE (in-gel western)^[7]

Specification

Features

• More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
• High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
• Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
• Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
• A non-antibody approach with no species restrictions against any co-staining antibody
• Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
• Stable in solution under 4 °C, eliminating the need to aliquot for storage 

User Guide with FAQ

For research use only. Not intended or approved for diagnostic or therapeutic use.

We do not accept returns of any kind.

Citation (119 total, selected listed)

• Johnson, Rebecca M., et al. "Investigating the dose-dependency of the midgut escape barrier using a mechanistic model of within-mosquito dengue virus population dynamics." PLoS Pathogens 20.4 (2024): e1011975.
• Yu, Gaoxiang, et al. "Evaluating the pro-survival potential of apoptotic bodies derived from 2D-and 3D-cultured adipose stem cells in ischaemic flaps." Journal of Nanobiotechnology 22.1 (2024): 333.
• Tran, Jeremy C., et al. "Fluorescein-Based SynNotch Adaptors for Regulating Gene Expression Responses to Diverse Extracellular Cues." bioRxiv (2024): 2024-06.
• J Neuroinflammation. 2024 Oct 1;21(1):247. doi: 10.1186/s12974-024-03229-x.
• Pedaprolu, Krishna. "Drivers of Fatigue Induced Degeneration in a Mouse Achilles Tendon Explant Model." Order No. 31085773 The Pennsylvania State University, 2023.
• Saxena, Pragya. Mas-Related G Protein-Coupled Receptor B2 Activation Mediates Bladder Wall Biomechanics. Diss. Michigan State University, 2024.
• Yuan, Yong, Yen-Chiao Wang, and Winston Kao. "Tbr1/Smad4 double knockout demonstrated that Smad4 played a pivotal role in Tbr1-dependent ECM synthesis and Tbr1-independent ECM degradation for maintaining corneal homeostasis." Investigative Ophthalmology & Visual Science 65.7 (2024): 3647-3647.
• Pertoll, Johanna. "Analysis of the collagen matrix in periodontal Ehlers-Danlos syndrom." (2024).
• Dong, Chengji, et al. "Zingerone promotes the survival of ischaemic flap via the KEAP1-Nrf2 pathway." Journal of Functional Foods 119 (2024): 106328.