2 × Phanta Flash Master Mix (Dye Plus) P520

Product introduction

2 × Phanta Flash Master Mix utilizes the new generation of Phanta Flash Super-Fidelity DNA Polymerase. Through the directed evolution of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of high fidelity (mismatch rate is 1/106 of conventional Taq DNA polymerase) and rapid amplification (5 sec/kb). With the application of the latest hot-start technology and the addition of specific factors, its amplification specificity and success rate are significantly improved. 2 × Phanta Flash Master Mix contains Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer. It only requires the addition of primers and templates for amplification, thereby reducing pipetting operations andimproving detection throughput and reproducibility of results.This product contains tracking dyes, and amplification products can be directly loaded for electrophoresis after the reaction. The amplification products of 2 × Phanta Flash Master Mix (Dye Plus) are blunt-ended, which are subsequently applicable for ClonExpress Ultra One Step Cloning Kit V2 (Vazyme #C116) and Ultra-Universal TOPO Cloning Kit (Vazyme #C603).

Features  

High fidelity: ~106X higher than Taq DNA Polymerase

Flash amplification: Extension time up to 5 sec/kb

Robust performance: Superior specificity, sensitivity, and yields

Broad Compatibility: Suitable for complex samples & GC-rich systems

Experimental Cases  

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤0°C.

Product Manual 

Selected recently Citations (total 56 citations)

1: Efficient Broad-Spectrum Cyanophage Function Module Mining; Yujing Guo, Xiaoxiao Dong, ..., Alexander Machado Cardoso; Microorganisms  |  2024 Aug 02 

2:A CRISPR-Cas9-Mediated Large-Fragment Assembly Method for Cloning Genomes and Biosynthetic Gene Cluster; Yujing Guo, Guang Cai, ..., Jin Wang; Microorganisms  |  2024 Jul 18