5 min TA/Blunt-Zero Cloning Kit C601

Product Description  

5 min TA/Blunt-Zero Cloning Kit is a second generation TOPO cloning kit that contains a second generation Topoisomerase, a vector containing the suicide gene ccdB and blunting factors. The second-generation Topoisomerase is matched with the optimal buffer, which has higher activity and further improves the cloning efficiency. Efficient ligation can be achieved by reacting at room temperature for 5 min. The vector contains the suicide gene ccdB. When the insert is successfully connected to the vector, the expression of the ccdB gene will be disrupted, and the host cell can grow normally, otherwise the host cell will die, thus realizing the "Zero" background. The blunting factor makes this product compatible with both TA cloning and blunt-end cloning.

Features  

Compatible: Suitable for sticky ends, blunt ends, TA , linker or adapter ligation

Fast: Cloning of either sticky- or blunt-end DNA in only 5 minutes

Efficient: High efficiency cloning and positive rate >95%

Components  

Storage  

Store at -30 ~ -15°C and transport at ≤0°C.

Product Manual

Selected Citations (total 140 citations)

1: The RING-Type E3 Ubiquitin Ligase Gene GhDIRP1 Negatively Regulates Verticillium dahliae Resistance in Cotton ( Gossypium hirsutum ), Fenglin Miao, Wei Chen, Songbiao Chen Plants  |  2024 Jul 25

2: A novel hypovirulence-associated Hadaka virus 1 (HadV1-LA6) in Fusarium oxysporum f. sp. cubense .Yinfu Lin, Guangqun Pan, Wenxia Fang, mSphere  |  2024 Jul 16  |  PubMed ID: 39012104 

3: A down-regulated cytochrome P450 in Neoseiulus barkeri Hughes (Acari: Phytoseiidae) can dechlorinate and hydroxylate chlorpyrifos without producing chlorpyrifos-oxon. Shi-Jiang Yu, Li Wang,  Chun Ran, Journal of hazardous materials  |  2024 Jul 09  |  PubMed ID: 38996679