TUNEL BrightRed Apoptosis Detection Kit A113

During apoptosis, intracellular specific endonucleases are activated, chromatin DNA is specifically cleaved between nucleosomes, and DNA is degraded into integer multiples of about 180 - 200  bp  fragments. The  3'-hydroxyl  (3'-OH)  end  generated  by  DNA  fragmentation  can  bind  to  fluorescein-12-deoxyuridine triphosphate (FITC-12-dUTP) under the action of terminal deoxynu-cleotidyl  transferase  (TdT). FITC-12-dUTP-labeled  DNA  can  be  directly  observed  by  fluorescence microscopy or quantitatively analyzed by flow cytometry to reflect the level of apoptosis. The BrightGreen  Labeling  Mix  contains  FITC-12-dUTP  and  Bright  factors. This  unique  small  molecule compound can non-covalently bind to FITC, enhance its stability and amplify its signal, resulting in brighter labeled fluorescence and stronger anti-quanching ability.

The patented Bright factor makes the fluorescence brighter and the anti-quenching ability stronger;
Label red fluorescence, which is convenient for co-labeling with other green fluorescence such as GFP;
Highly active recombinant TdT enzyme ensures the efficiency of fluorescence incorporation;
Suitable for cell slides, paraffin sections and frozen sections

Store at -20℃ for up to 12 months; Store BrightRed Labeling Mix at -20℃ in the dark