ADP Colorimetric/Fluorometric Assay Kit

Description

ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.

Tribioscience’s ADP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method in which ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (Absorbance = 570 nm) or fluorometric method (Ex/Em 530/590 nm). The assay is simple, sensitive, stable, and high-throughput adaptable. The assay can detect as low as 1 µM ADP in various biological samples.

Applications

Direct Assays: As low as 1 µM of ADP in cells and other biological samples.

Key Features

• Sensitive and accurate: Use 10 µL samples. Detection range 1-1000 µM in a 96-well plate assay.
• Simple: Just load and incubate procedure; takes less than 60 minutes.
• High-throughput: Kit is designed to be a robust method for High-throughput manner.

Storage Conditions

Store kit at -20°C. Shelf life of three months. Except Enzymes, warm all the components to room temperature before use. Briefly centrifuge all small vials prior to opening.

Standard Curve