NNMT Activity Assay (Colorimetric / Fluorometric) (TBS2098)

Description

NNMT (Nicotinamide N-Methyltransferase) is an enzyme that catalyzes the methylation of nicotinamide (NA) and other pyridines using S-adenosyl-L-methionine (SAM) as the methyl group donor to produce SAH and 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways. It is expressed at high levels in several kinds of cancers, neurodegenerative diseases, obesity, and diabetes, indicating a potential target for therapy.

Tribioscience’s NNMT Activity Assay utilizes SAM and nicotinamide as the substrates to generate SAH which is hydrolyzed to form homocysteine. The free thiol group of the homocysteine can be detected with a thiol probe generating an enhanced fluorescent signal at Ex/Em = 400/465 nm. The assay principle is displayed in Figure 1. The kit provides the easiest and most accurate approach to measure NNMT activity in a variety of samples.

Application

Measure NNMT activity in a variety of samples.

Fig. 1: Principle of NNMT Assay

Fig. 2: Standard Curve