Product Introduction
2 × Rapid Taq Master Mix contains Taq DNA Polymerase, elongation promoting factor, dNTP and an optimized buffer system. The amplification speed of this product can reach 15 sec/kb, which is suitable for rapid PCR. The maximum amplification speed within 1 kb can reach 1 sec/kb, greatly saving reaction time. The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The kit has excellent amplification performance and high storage stability. It is suitable for PCR amplification within 5 kb using genome as template and PCR amplification within 10 kb using plasmid and λDNA as template. The protective agent added to the system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. This product contains loading buffer and tracking dye, so PCR products can be directly loaded for electrophoresis after the reaction, which is convenient to use. The PCR product has an adenine at the 3' end that can be cloned into the T vector. It is compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C116/C603).
Features
Rapid amplification: 15 sec/kb; extension time up to 1 sec/kb (≤ 1kb fragments)
Convenience: Include tracking dye for direct loading of PCR products on gels
Good stability: Stable performance after 50 freeze-thaw cycles
Ready-to-use: Simplified workflow and minimized pipetting steps with 2 × Master Mix format
Components
Storage
Store at -30 ~ -15°C and transport at ≤0°C. It can be stored at 4°C for 3 months after thawing.
Selected Citation (346 citations total)
1: CD131 Contributes to Ulcerative Colitis Pathogenesis by Promoting Macrophage Infiltration,Wu Zhiyuan, Liu Lindi, ..., Tan Xiaodong bioRxiv | 2024 Aug 09
2: Unveiling potential sex-determining genes and sex-specific markers in autotetraploid Carassius auratus.Kun Zhang, Xu Huang, ..., Shaojun Liu
Science China. Life sciences | 2024 Aug 09 | PubMed ID: 39136860
3: Fruiting Body Heterogeneity, Dimorphism and Haustorium-like Structure of Naematelia aurantialba (Jin Er Mushroom),Ying Yang, Caihong Dong, Zuzanna Magdziak, Journal of Fungi | 2024 Aug 07 | PubMed ID: 39194883
