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Product Description
Description
Tribioscience’s Fast ACTH ELISA is designed to quantitatively detect ACTH levels in serum, plasma, and other biological samples with our FAST proprietary approaches to combine samples and detections into a one-step. It makes the assay simple, easy, accurate, and fast. The total operating time can be finished within 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 7 to 500 pg/mL. The ACTH levels of samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for ATCH.
Typical Data
This standard curve(R2=0.999) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of ACTH is typically 5 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human ACTH. No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human ACTH Microplate
TBS3290A
96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for ACTH.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human ACTH Standard
TBS3290B
60 µl of Recombinant ACTH (10 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3290C
2.1 ml of ACTH Detection antibody.
May be stored for up to 3 months at 2-8 °C.
Assay Diluent
TBS3290D
12 ml of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit is sufficient for one 96-well plate ELISA.
Fast Adrenocorticotropic Hormone / ACTH ELISA, 1step and 1hr (TBS3290)
$560.00
Unit price /
Unavailable
Description
Tribioscience’s Fast ACTH ELISA is designed to quantitatively detect ACTH levels in serum, plasma, and other biological samples with our FAST proprietary approaches to combine samples and detections into a one-step. It makes the assay simple, easy, accurate, and fast. The total operating time can be finished within 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 7 to 500 pg/mL. The ACTH levels of samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for ATCH.
Typical Data
This standard curve(R2=0.999) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of ACTH is typically 5 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human ACTH. No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human ACTH Microplate
TBS3290A
96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for ACTH.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human ACTH Standard
TBS3290B
60 µl of Recombinant ACTH (10 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3290C
2.1 ml of ACTH Detection antibody.
May be stored for up to 3 months at 2-8 °C.
Assay Diluent
TBS3290D
12 ml of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit is sufficient for one 96-well plate ELISA.
