Tribioscience’s Fast Human Tau (Total) ELISA is designed to quantitatively detect human Tau (Total) levels in CSF, serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8000 to 125 pg/mL. The levels of human Tau (Total) protein in samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human Tau (Total) protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human tau total is typically 200 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human tau total.
No cross-reactivity with others.
Simple Procedures
Simple Standard Preparation
Kit Content and Storage Conditions
| Part |
Part# |
Description |
Storage of Opened/Reconstituted |
| Human Tau (Total) Microplate |
TBS3295A |
96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human Tau (Total). |
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human Tau (Total) Standard |
TBS3295B |
30 µl of Recombinant human Tau (Total) (100 ng/mL). |
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A |
TBS3295C |
2.1 ml of human Tau (Total) Detection antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B |
TBS3295D |
400 µL of Streptavidin-HRP. |
| Assay Diluent |
TBS3295E |
25 mL of a buffered protein base with preservatives. |
| Wash Buffer |
TBS3000W |
12 mL of concentrated solution (10x). |
| TMB Substrate |
TBS3000T |
12 mL of ultra-sensitive TMB substrate. |
| Stop Solution |
TBS3000S |
6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Related Product
- Human IL-1ß ELISA (TBS3219)
- Human IL-2 ELISA (TBS3220)
- Human IL-4 ELISA (TBS3221)
- Human IL-6 ELISA (TBS3223)
- Human IL-7 ELISA (TBS3224)
