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Product Description
Description
The Fast Mouse C3d ELISA is a solid phase ELISA designed to measure mouse C3d levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours, not need 4-5 hours (Fig. 1). The detection range is from 1 to 250 ng/mL. The levels of mouse C3d samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse C3d protein.
Typical Data
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical data.
Sensitivity
The minimum detectable dose (MOD) of mouse C3d is typically 1 ng/ml.
The Intra-assay CV and the Inter-assay CV are < 10%.
Specificity
This assay recognizes natural and recombinant mouse C3d.
No cross reaction: Human C3d.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Mouse C3d Microplate
TBS3022A
96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for mouse C3d.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Mouse C3d Standard
TBS3022B
20 µl of Recombinant mouse C3d protein (10 µg/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3022C
2.1 ml of mouse C3d antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3022D
12 ml of Streptavidin-HRP.
Assay Diluent
TBS3022E
12 ml of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
The Fast Mouse C3d ELISA is a solid phase ELISA designed to measure mouse C3d levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours, not need 4-5 hours (Fig. 1). The detection range is from 1 to 250 ng/mL. The levels of mouse C3d samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse C3d protein.
Typical Data
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical data.
Sensitivity
The minimum detectable dose (MOD) of mouse C3d is typically 1 ng/ml.
The Intra-assay CV and the Inter-assay CV are < 10%.
Specificity
This assay recognizes natural and recombinant mouse C3d.
No cross reaction: Human C3d.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Mouse C3d Microplate
TBS3022A
96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for mouse C3d.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Mouse C3d Standard
TBS3022B
20 µl of Recombinant mouse C3d protein (10 µg/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3022C
2.1 ml of mouse C3d antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3022D
12 ml of Streptavidin-HRP.
Assay Diluent
TBS3022E
12 ml of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
