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Product Description
Description
Tribioscience’s Human GFAP ELISA is designed to quantitatively detect human GFAP levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 4 to 3000 pg/mL. The levels of human GFAP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human GFAP protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human is typically 4 pg/mL. The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human GFAP. No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human GFAP
Microplate
TBS32106A
96 well polystyrene microplate (12 strips of 8 wells) coated
with a monoclonal antibody specific for human GFAP.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human GFAP
Standard
TBS32106B
100 µl of Recombinant human GFAP (30 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS32106C
2.1 mL of biotin- human GFAP antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS32106D
200 µL of streptavidin HRP.
Assay Diluent
TBS32106E
25 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Tribioscience’s Human GFAP ELISA is designed to quantitatively detect human GFAP levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 4 to 3000 pg/mL. The levels of human GFAP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human GFAP protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human is typically 4 pg/mL. The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human GFAP. No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human GFAP
Microplate
TBS32106A
96 well polystyrene microplate (12 strips of 8 wells) coated
with a monoclonal antibody specific for human GFAP.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human GFAP
Standard
TBS32106B
100 µl of Recombinant human GFAP (30 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS32106C
2.1 mL of biotin- human GFAP antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS32106D
200 µL of streptavidin HRP.
Assay Diluent
TBS32106E
25 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
