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Product Description
Description
The Fast Human IL-17A ELISA is a solid phase ELISA designed to measure human IL-17A levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not 5-6 hours (Fig. 1). The detection range is from 8 to 2000pg/mL. The levels of human IL-17A samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-17A protein.
Typical Data
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human IL-17A is typically 6.5 pg/ml. The Intra-CV is 5.39%, and the Inter-CV is < 12%.
Specificity
This assay recognizes natural and recombinant human IL-17A.
No-cross reactivity: Human INF-γ, IL-10, IL-12, IL-16, IL-17B, IL-17C, IL-17D, IL-17F; Mouse IL-17.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human IL-17A
Microplate
TBS3228A
96-well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for human IL-17A.
The unused wells can be stored in the sealed foil pouch
containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-17A
Standard
TBS3228B
60 μL of Recombinant human L-17A protein (100ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost
freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3228C
2.2 mL of human IL-17A antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3228D
120 μL of Streptavidin-HRP.
Assay Diluent
TBS3228E
12 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use an expired kit for assay.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
The Fast Human IL-17A ELISA is a solid phase ELISA designed to measure human IL-17A levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not 5-6 hours (Fig. 1). The detection range is from 8 to 2000pg/mL. The levels of human IL-17A samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-17A protein.
Typical Data
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human IL-17A is typically 6.5 pg/ml. The Intra-CV is 5.39%, and the Inter-CV is < 12%.
Specificity
This assay recognizes natural and recombinant human IL-17A.
No-cross reactivity: Human INF-γ, IL-10, IL-12, IL-16, IL-17B, IL-17C, IL-17D, IL-17F; Mouse IL-17.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human IL-17A
Microplate
TBS3228A
96-well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for human IL-17A.
The unused wells can be stored in the sealed foil pouch
containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-17A
Standard
TBS3228B
60 μL of Recombinant human L-17A protein (100ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost
freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3228C
2.2 mL of human IL-17A antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3228D
120 μL of Streptavidin-HRP.
Assay Diluent
TBS3228E
12 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use an expired kit for assay.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
