Human IL-2 ELISA; 1 step and 1hr

Description

The Fast Human IL-2 ELISA is a solid-phase ELISA designed to measure human IL-2 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human IL-2 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-2 protein.

Key Features

• Novel Proprietary ELISA Approach
• Fast: Only 1 hour, not 4-5 hrs.
• Simple: Sample and Detection combined into one step.
• Easy: All procedures are performed at room temperature.
• High Sensitivity: The lowest detection limit is 8.0 pg/ml.

Principle of the Assay

Kit Content and Storage Condition

Typical Data

This standard curve(R²=0.9991) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical data.

Sensitivity

The minimum detectable dose (MOD) of human IL-2 is typically 10 pg/ml.

The Intra-assay CV is 5.45% the Inter-assay CV is 8.6%.

Specificity

This assay recognizes natural and recombinant human IL-2.

No cross reaction: Human IL-2 Rα, Human IL-2 Rβ, Human IL-2 Rγ, Mouse IL-2

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