Human IL-7 ELISA; 1 step and 1hr

Description

Interleukin-7(IL-7), is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells.

Key Features

• Novel Proprietary ELISA Approach
• Fast: Only 2 hr, not 5-6 hrs.
• Simple: Sample and Detection combined into one step.
• Easy: All procedures are performed at room temperature.
• High Sensitivity: The low detection limit is 3.0pg/mL.

The levels of human IL-7 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-7 protein.

Principle of Assay

Kit Content and Storage Condition

Typical Data

This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of human IL-7 is typically 3.2 pg/ml.

The Intra-CV is 4.76% and the Inter-CV is 7.12 %.

Specificity

This assay recognizes natural and recombinant human IL-7.

No cross-reactivity: Human IL-7 R; Mouse IL-7; Mouse IL-7 Rα.