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Product Description
Description
Human Menin ELISA is a solid phase ELISA designed to measure human Menin levels in cell culture supernatants, serum, plasma, and cell lysates. The main feature is that the kit makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours, not 5-6 hours (Fig. 1), and only includes one washing step before color development. The detection range is from 1.638 to 400 ng/mL. The levels of human Menin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for human Menin protein.
Typical Data
This standard curve(R2=1.000) is provided in Fig. 3 for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human Menin is typically 0.6 ng/ml.
The Intra-CV is < 10%, and the Inter-CV is < 10%.
Specificity
This assay recognizes natural and recombinant human Menin.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human Menin Microplate
TBS32100A
96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human Menin.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human Menin Standard
TBS32100B
60 μL of Recombinant human Menin protein (20ug/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS32100C
12 mL of human biotinylated Menin antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS32100D
12 mL of Streptavidin-HRP
Assay Diluent
TBS32100E
12 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Human Menin ELISA is a solid phase ELISA designed to measure human Menin levels in cell culture supernatants, serum, plasma, and cell lysates. The main feature is that the kit makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours, not 5-6 hours (Fig. 1), and only includes one washing step before color development. The detection range is from 1.638 to 400 ng/mL. The levels of human Menin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for human Menin protein.
Typical Data
This standard curve(R2=1.000) is provided in Fig. 3 for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human Menin is typically 0.6 ng/ml.
The Intra-CV is < 10%, and the Inter-CV is < 10%.
Specificity
This assay recognizes natural and recombinant human Menin.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human Menin Microplate
TBS32100A
96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human Menin.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human Menin Standard
TBS32100B
60 μL of Recombinant human Menin protein (20ug/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS32100C
12 mL of human biotinylated Menin antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS32100D
12 mL of Streptavidin-HRP
Assay Diluent
TBS32100E
12 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
