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Tribo™ Mouse Granzyme B ELISA Kit
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Tribioscience’s Fast Mouse Granzyme B ELISA is designed to quantitatively detect mouse Granzyme B levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 7 to 500 pg/mL. The levels of mouse Granzyme B samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse Granzyme B protein.
This standard curve (R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
The minimum detectable dose (MOD) of mouse Granzyme B is typically 13 pg/ml.
The Intra-assay CV is 3.79%, the Inter-assay CV is ˂10%.
This assay recognizes natural and recombinant mouse Granzyme B.
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Mouse Granzyme B Microplate | TBS3083A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for mouse Granzyme B. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Mouse Granzyme B Standard | TBS3083B | 30 µL of Recombinant mouse Granzyme B protein (25 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3083C | 2.2 mL of Biotin-mouse Granzyme B antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3083D | 300 µL of Streptavidin-HRP. | |
| Assay Diluent | TBS3083E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
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