Keratinocyte chemoattractant (KC), also known as CXCL1, SCYB1, or N51 is a member of the CXC or alpha chemokine family. KC is similar in structure and activity to IL-8 and MIP-2. It is produced by melanocytes, epidermal keratinocytes, monocytes, macrophages, mammary epithelial cells, endothelial cells, neutrophils, fibroblasts, and hepatocytes. The expression level of this chemokine is normally very low in most tissues however, it can be significantly elevated in many disease states including rheumatoid arthritis, cancer, experimental autoimmune encephalopathy, post surgery, and other inflammatory responses.
The Tribo™ Mouse KC ELISA Kit is designed to detect mouse KC levels quantitatively in different tissues including skin, muscle, neural, serum, and other biological samples. The plate has been coated with murine KC monoclonal antibody. Any KC present in the sample or standard will be captured. The procedure calls for a biotin-conjugated anti-murine KC antibody to be added along with streptavidin-horseradish peroxidase (HRP) with an accompanying substrate. The reaction exhibits a blue color in direct proportion to the amount of KC in the initial sample. The reaction is terminated by the addition of a stop solution and absorbance is measured at 450nm. The concentration of KC in the samples is calculated opposite to the standard curve.
Applications
Direct Assays: quantitative KC in plasma and serum.
Key Features
- Sensitivity: The minimum detectable dose of mouse KC is less than 8.0pg/mL typically.
- Specificity: This assay recognizes recombinant and natural mouse KC. No cross-reactivity with any of the other cytokine is observed.
Kit Contents
| Reagents | Quantity |
|---|---|
| Precoated 96-well Plate (8x 12 strips) | 1 Plate |
| Standard | 2x 100μL |
| Detection A | 1x 100μL |
| Detection B | 1x 100μL |
| Sample Diluent (10x Concentrate) | 1x 12mL |
| TMB Substrate Solution | 1x 12mL |
| Stop Solution | 1x 12mL |
| Wash Solution (20x Concentrate) | 1x 50mL |
| Plate Sealers | 4 |
Storage Conditions
Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receipt.
