TWEEN® 20
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TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
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| Application | Washing buffer and blocking buffer in Western blotting |
|---|---|
| Blocking buffer in immunohistochemistry | |
| Washing buffer in ELISA | |
| Reaction mixture in PCR |
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