2-Phosphoglycerate Colorimetric / Fluorometric Assay (TBS2206)

Description

2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2PG is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research.

Tribioscience’s 2-Phosphoglycerate Assay kit provides a sensitive, fast, and easy-to-use approach. In this assay, 2PG is converted by Enzyme Mix to PEP, which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to the 2PG level. This assay kit can detect 2PG levels below 20 pmol and can be used for a variety of sample types.

FEATURES

• Flexible: Can be used 96 well-plate or 384 well-plate with the colorimetric and fluorometric assay.
• Accuracy: Absorbance and Fluorescence intensity is proportional to the 2PG level.

APPLICATIONS

• Measurement of 2-Phosphoglycerate in various tissues/cells.
• Analysis of carbohydrate metabolism and cell signaling.
• Cancer research.

TYPICAL DATA