8-pCPT-2-O-Me-cAMP-AM, Epac activator (TBI2736)

Description

Product Details

Formal Name: 8-(4-Chlorophenylthio)-2’-O-methyladenosine-3’,5’-cyclic monophosphate, acetoxymethyl ester

Molecular Formula: C₂₀H₂₁ClN₅O₈PS

Formula Weight: 557.91

CAS Number: 1152197-23-3

Purity: >98%

Formulation: powder

Solubility: Soluble in DMSO (up to 50 mg/ml).

Storage: -20°C

Stability: ≥ 2 years.

Applications

Epac activator

Functions

A potent, cell-permeable Epac (exchange protein directly activated by cAMP) activator. Induces RAP1 activation and insulin secretion in pancreatic beta cell lines. Induces vascular relaxation in rat mesenteric artery. The acetoxymethyl ester confers increased cell-permeability and is cleaved by endogenous esterases to yield the active compound, 8-pCPT-2′-O-Me-cAMP. Addition to cell cultures should be done in serum-free media as esterases in serum will cleave the acetoxymethyl ester and reduce cell permeability.

Application Procedures

First dissolved in DMSO (up to 50 mg/ml), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20°C for up to 1 month.