Accutase Cell Detachment Solution

Description

Accutase is a cell detachment solution comprised of collagenolytic and proteolytic enzymes that are not mammalian or bacterial derived. Accutase is a replacement for trypsin-EDTA solution. It performs exceptionally well for detaching cells for continued cell culturing, the analysis of cell surface markers using flow cytometry, and for cell sorting. Accutase has been demonstrated effective in detaching multiple cell types including human and mouse embryonic and induced pluripotent stem cells, human neural stem cells, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, neural cell types, bone marrow derived stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.

Components

• Accutase Enzymes
• EDTA
• Phenol Red
• PBS
• pH: 7.2-80