Acetaldehyde Assay Kit (Fluorometric) (TBS2094)

Description

Acetaldehyde is one of the most widely occurring aldehydes in nature. In the liver, ethanol is first converted to acetaldehyde by alcohol dehydrogenase. Acetaldehyde is toxic to the human body and is rapidly converted to the less harmful acetic acid by aldehyde dehydrogenase. Acetaldehyde is more toxic than alcohol and is responsible for many hangover symptoms. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and result in facial and body flushing referred to as “Asian flush syndrome”. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine.

Tribioscience’s Acetaldehyde Assay Kit is a simple and fast assay to measure the total amount of acetaldehyde in biological samples. Acetaldehyde is oxidized by aldehyde dehydrogenase and the generated NADH reduces a specific probe to a final product measured at Ex/Em=530nm/600 nm. The fluorescent intensity is proportional to the acetaldehyde concentration in the sample.

Applications

• Measure acetaldehyde in a variety of samples.

Storage Conditions

Store the Reagent at -20°C and protect it from light. Store Acetaldehyde Standard at 4°C.

Standard Curve