Adenosyl homocysteinase Fluorometric Assay (TBS2056)

Description

Adenosylhomocysteinase (AHCY) or S-adenosylhomocysteine hydrolase (SAHH) is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine (Hcy). In mammals, AHCY is the only enzyme capable of performing this reaction. SAH is the by-product and a potent inhibitor of methyltransferase activity. AHCY deletion is embryonic lethal in many organisms (from plants to mammals). In humans, AHCY deficiency is associated with an incurable rare recessive disorder in methionine metabolism.

Tribioscience’s AHCY Activity Assay utilizes SAH as the substrate to form homocysteine and adenosine. The free thiol group of the homocysteine can be detected with a thiol probe generating an enhanced fluorescent signal at Ex/Em = 400/465 nm. The assay principle is displayed in Figure 1. The kit provides the easiest and most accurate approach to measuring AHCY activity in a variety of samples.

Fig. 1: Principle of AHCY Assay

Applications

• Measure AHCY activity in a variety of samples.

Storage Conditions

Store the Reagent at –20° (–80°C for AHCY Enzyme positive control), protected from light.

Shelf life: 6 months.

Standard Curve