ALP Fluorescence Substrate system

Description

4-Methylumbelliferyl Phosphate (4-MUP) is a substrate for an alkaline phosphatase that forms the soluble highly fluorescent reaction product methylumbelliferone. The 4-MUP Liquid Substrate System combines 4-Methylumbelliferyl Phosphate (4MUP) and buffer in a single solution for detecting alkaline phosphatase enzyme activity in the culture media or ELISA. Fluorescence can be measured with excitation at 360 nm and emission at 450 nm. Also, the fluorescent product may also be observed using a UV light source. This substrate is about 10 times more sensitive than the alkaline phosphatase substrate BCIP/NBT.

Features

• Flexible: Can be used for 96-well and 384-well plate.
• Sensitivity: More sensitive than another alkaline phosphatase substrate like BCIP/NBT.
• Time-saving: Just add and read manner.

Applications

• ALP substrate – for detection of alkaline phosphatase (alk-phos) activity on the liquid system.
• Used for immunoassays like ELISA and western blot.
• Custom packaging and bulk purchase information is available upon request.

Storage Conditions

Store at -20°C and protect from light for up to 2 years.