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GenDEPOT  |  SKU: P0322-010

amfiSure Hot-Start Taq DNA Polymerase

$78.00
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GenDEPOT

amfiSure Hot-Start Taq DNA Polymerase

From $78.00
amfiSure Hot-Start Taq DNA Polymerase is designed for hot start PCR, a technique that enhances the specificity, sensitivity and yield of DNA amplification. In addition, the enzyme provides the convenience of reaction set-up at room temperature. The Hot Start Taq DNA Polymerase is a recombinant Taq DNA Polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. 

The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers. The functional activity of the enzyme is restored during a short 4-min incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA Polymerase: it catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' proofreading exonuclease activity, but possesses low 5'→3' exonuclease activity.

Store at -20℃

Contents amfiSure Hot-Start Taq DNA Polymerase, 5u/ul
amfiSure 10X Hot-Start PCR Buffer
Storage Buffer 20mM Tris-HCl(pH 9.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.5% Tween 20 and 50% glycerol

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