Boron Colorimetric Assay

Description

Boron is an essential micronutrient in plants and is involved in maintaining robust cell walls, cell membranes, and reproductive tissues. Although boron is common in the soil in its natural state as a borate mineral, the amount of boron available to plants is quite small. As a result, boron deficiency is the second most common micronutrient deficiency among crop plants. In order to keep plant boron levels in a healthy range, supplementation to the soil via fertilizers and additives is often required. If not regulated, a lack or excess of boron may significantly lower crop yield. In the biotech industry, sodium borohydride is commonly used to conjugate antibodies and typically needs to be removed from the final product, especially for therapeutic antibodies.
Tribioscience’s Boron Colorimetric Assay provides a convenient and reliable means to measure boron. In the assay, borate complexes with azomethine-H to create a colored compound that can be measured at 420nm. This assay can be used with a variety of samples and is simple, sensitive, and adaptable to high-throughput screening.

Applications:

Direct Assays: Boron in water, plant tissues, soil samples, and antibody conjugation solutions.

Key Features:

Fast: Just one step that takes less than 10 minutes.
Accurate: Linear detection range of 0.1-20 μg/mL (0.1-20 ppm) boron in 50μL samples.
Simple and High-Throughput: The reagents are ready to use. The kit can be used as a robust method for a high-throughput 96-well or 384-well plate.

Kit Contents (for 200 Assays):

Storage and Handling:

Store kit at -20℃.
Shelf life of six months.

Example Standard Curve