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Product Description
Description
Chloramphenicol is a broad-spectrum antibiotic effective against a wide variety of Gram-positive and Gram-negative bacteria. It is no longer commonly prescribed to patients because of the presence of resistant bacteria and safety concerns. One of its most prominent adverse effects is bone marrow toxicity, which includes direct bone marrow suppression and aplastic anemia, with the latter being relatively rare but unpredictable, not dosage-dependent, and generally fatal. Chloramphenicol is especially toxic for neonates. However, due to its low cost and effectiveness to improve yield, Chloramphenicol has been illegally used in agriculture and therefore is present in some seafood such as shrimp or crawfish. There are reports on its presence in milk, honey, and meat products as well.
Tribioscience’s Chloramphenicol Fast ELISA Kit provides a flexible accurate, sensitive, and time-saving approach to determining the presence of Chloramphenicol in animal tissue (e.g. chicken, beef, fish, or shrimp), honey, or milk, providing a vital tool to prevent consumption of food tainted with Chloramphenicol.
Kit Components
Part
Part#
Description
Storage of Opened/Reconstituted
Pre-coated Microplate
TBS21121A
96 well microplate (12 strips of 8 wells) coated with an antibody specific for chloramphenicol.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Chloramphenicol Standard
TBS21121B
40 µL of chloramphenicol (81 ng/mL, 20x).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS21121C
80 µL of HRP- chloramphenicol conjugate (100x).
May be stored for up to 3 months at 2-8 °C.
Assay Diluent
TBS3000D
25 ml of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
10 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 1 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Chloramphenicol is a broad-spectrum antibiotic effective against a wide variety of Gram-positive and Gram-negative bacteria. It is no longer commonly prescribed to patients because of the presence of resistant bacteria and safety concerns. One of its most prominent adverse effects is bone marrow toxicity, which includes direct bone marrow suppression and aplastic anemia, with the latter being relatively rare but unpredictable, not dosage-dependent, and generally fatal. Chloramphenicol is especially toxic for neonates. However, due to its low cost and effectiveness to improve yield, Chloramphenicol has been illegally used in agriculture and therefore is present in some seafood such as shrimp or crawfish. There are reports on its presence in milk, honey, and meat products as well.
Tribioscience’s Chloramphenicol Fast ELISA Kit provides a flexible accurate, sensitive, and time-saving approach to determining the presence of Chloramphenicol in animal tissue (e.g. chicken, beef, fish, or shrimp), honey, or milk, providing a vital tool to prevent consumption of food tainted with Chloramphenicol.
Kit Components
Part
Part#
Description
Storage of Opened/Reconstituted
Pre-coated Microplate
TBS21121A
96 well microplate (12 strips of 8 wells) coated with an antibody specific for chloramphenicol.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Chloramphenicol Standard
TBS21121B
40 µL of chloramphenicol (81 ng/mL, 20x).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS21121C
80 µL of HRP- chloramphenicol conjugate (100x).
May be stored for up to 3 months at 2-8 °C.
Assay Diluent
TBS3000D
25 ml of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
10 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 1 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
