Equine Insulin Fast ELISA

Description

The Equine Insulin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of equine insulin in serum, plasma, and cell culture supernatants. The microplate is coated with mouse monoclonal antibody specific for Insulin. Standards or samples are pipetted into the wells and incubated together with biotinylated anti-insulin monoclonal antibody (Detection A). The insulin present in a sample is bound to the wells by the capture antibody, and then by biotinylated antibody, producing an antibody-antigen-antibody “sandwich”. After washing away unbound biotinylated antibodies, HRP-streptavidin (Detection B) is pipetted to the wells. The wells are again washed, and a TMB substrate solution is added to the wells and exhibits a blue color in direct proportion to the amount of insulin in the initial sample. The reaction is terminated by the addition of an acid, and absorbance is measured at 450 nm. The concentration of insulin in the samples is determined by comparing the O.D. of the samples to the standard curve.

Applications

Direct Assays: Insulin concentrations in different tissues.

Key Features

• Rapid assay: Less than 3 hours.
• Every reagent is provided in liquid form and ready to use.
• Excellent precision and reproducibility.
• A simple assay procedure without any pretreatment of samples.

Kit Contents

Storage Conditions

Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 6 months after receipt.