Tribioscience’s Fast Human Phospho Tau Thr217 (p-Tau217) ELISA is designed to quantitatively detect human p-Tau217 levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, no need for 4-5 hours (Fig. 1). The detection range is from 0.3 to 243 ng/mL. The levels of human p-Tau217 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human p-Tau217 protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human is typically 200 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human pTau-217.
No cross-reactivity with others.
Simple Procedures
Simple Standard Preparation
Kit Content and Storage Conditions
| Part |
Part# |
Description |
Storage of Opened/Reconstituted |
| Human PTau-217 Microplate |
TBS3293A |
96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human p-tau217. |
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human PTau-217 Standard |
TBS3293B |
50 µl of Recombinant human p-tau217 (2.43 μg/mL). |
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A |
TBS3293C |
2.1 ml of human p-tau217 Detection antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B |
TBS3293D |
20 µL of HRP conjugated secondary antibody. |
| Assay Diluent |
TBS3293E |
25 mL of a buffered protein base with preservatives. |
| Wash Buffer |
TBS3000W |
12 mL of concentrated solution (10x). |
| TMB Substrate |
TBS3000T |
12 mL of ultra-sensitive TMB substrate. |
| Stop Solution |
TBS3000S |
6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
