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TribioScience  |  SKU: TBS3232

Fast Human TGF-β1 ELISA

$450.00
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TribioScience

Fast Human TGF-β1 ELISA

$450.00

Description

The Fast Human TGF-β1 ELISA is a solid phase ELISA designed to measure human TGF-β1 levels in cell culture supernatants, serum, and plasma. The main feature of this kit is its use of a novel fast approach to replace traditional methods. The kit is designed to ensure short steps, a short incubation time, and only one set washing out. The measurement can be finished within 2 hours instead of the traditional 4-5 hours. The detection range is between 10-4,000 pg/mL. The levels of human TGF-β1 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TGF-β1 protein.

Kit Contents and Storage Conditions

Part Part# Description Storage of Opened/Reconstituted
Human TGF-β1 Microplate TBS3232A 96-Well Polystyrene Microplate (12-strips of 8-wells) coated with a capture antibody specific for human TGF-946;1. Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip seal. May be stored for up to 1 month at 2-8℃
Human TGF-β1 Standard TBS3232B 0.1mL of Recombinant human TGFβ1 protein (100 ng/mL). Aliquot and store at -20℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3232C 2.2mL of human TGF-β1 antibody. May be stored for up to 3 months at 2-8℃
Detection B TBS3232D 120μL of Streptavidin-HRP (100x).
Assay Diluent TBS3232E 12mL of buffered protein base with preservatives.
10x Wash Buffer TBS3000W 12mL of concentrated solution (10x).
TMB Substrate TBS3000T 12mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6mL of 2N sulfuric acid.

Store the unopened kit at 2-8℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Accurate Standard Curve

Example Standard Curve

Size

  • 1 Kit
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