GFP-Tag Mouse Monoclonal Antibody, 1mg/ml

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells.

GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues. GenDEPOT’s GFP-Tag Mouse Monoclonal Antibody recognizes both the native and denatured forms of GFP (Green Fluorescent Protein) and its many popular variants: EGFP, YFP, EYFP, and CFP.

Reactivity	Human
Clonality	Monoclonal , GF28R
Host	Mouse
Isotype	IgG2b
Form	Liquid, 1mg/ml in 10mM PBS, pH 7.2 with 0.05% NaN3
Application	WB : 1:1000 - 1:3000
	IF : 1:200 - 1:500
	IP : 1:50 - 1:200