HDAC colorimetric assay kit

Description

Histones are the chief protein components of chromatin, which act as spools around which DNA winds. Covalent modification of histone proteins through acetylation and deacetylation affects chromatin structure and regulates gene expression. Histone hyperacetylation is well correlated with increased transcription, whereas hypoacetylation correlates with transcriptional repression. Histone deacetylases (HDACs) catalyze the removal of acetyl groups from an ɛ-N-acetyl lysine amino acid on a histone, and act as transcriptional repressors of genes. Histone deacetylases have been grouped into three classes. Class I (HDAC 1, 2, 3, 8) and Class II (HDAC 4, 5, 6, 7, and 9) are zinc-containing hydrolase enzymes. Class III of deacetylases consists of the members of the sirtuin family of enzymes (Sir 1 to 7). Inhibitors of HDAC classes II and I are being studied as a treatment for cancer and neurodegenerative diseases such as Huntington’s and Alzheimer’s diseases. The Sirtuin 1 (class III) enzyme represents a target for the treatment of age-related diseases and type II diabetes.

The Tribo^TM HDAC Colorimetric Assay Kit provides a convenient, sensitive, and simple approach to quantify HDAC activity from various sources of enzyme using a colorimetric substrate. In the HDAC assay, an acetylated substrate is incubated with HDAC-containing samples. Deacetylation of the substrate sensitizes it in the presence of HDAC developer, and generates the colorimetric substrate, which can be detected at OD400nm. The substrate included in the kit is cell-permeable, and the assay can measure HDAC activity directly in cell culture in a 96-well plate without a time-consuming cell extraction step. The kit also can be used for HTS of HDAC inhibitors with extracts or purified enzymes.

Storage Conditions

Store the substrate at -20°C protected from light. The other components may be stored at 4°C. Shelf life: 12 months.