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Product Description
Description
The Fast Human IL-10 ELISA is a solid phase ELISA designed to measure human IL-10 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not 5-6 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human IL-10 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-10 protein.
Typical Data
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human IL-10 is typically 6.5 pg/ml. The Intra-CV is 5.39%, and the Inter-CV is < 12%.
Specificity
This assay recognizes natural and recombinant human IL-10.
No-cross reactivity: Human IL-10 Rα; Human IL-10 Rβ/Fc Chimera; Human IL-22; Human IL-24; rat IL-10; feline IL-10; mouse IL-10; porcine IL-10.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human IL-10
Microplate
TBS3226A
96-well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for human IL-10.
The unused wells can be stored in the sealed foil pouch containing
the desiccant pack for up to 1 month at 2-8 °C.
Human IL-10
Standard
TBS3226B
60 μL of Recombinant human IL-10 protein (100ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3226C
2.2 mL of human IL-10 antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3226D
120 μL of Streptavidin-HRP.
Assay Diluent
TBS3226E
12 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
The Fast Human IL-10 ELISA is a solid phase ELISA designed to measure human IL-10 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not 5-6 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human IL-10 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-10 protein.
Typical Data
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human IL-10 is typically 6.5 pg/ml. The Intra-CV is 5.39%, and the Inter-CV is < 12%.
Specificity
This assay recognizes natural and recombinant human IL-10.
No-cross reactivity: Human IL-10 Rα; Human IL-10 Rβ/Fc Chimera; Human IL-22; Human IL-24; rat IL-10; feline IL-10; mouse IL-10; porcine IL-10.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human IL-10
Microplate
TBS3226A
96-well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for human IL-10.
The unused wells can be stored in the sealed foil pouch containing
the desiccant pack for up to 1 month at 2-8 °C.
Human IL-10
Standard
TBS3226B
60 μL of Recombinant human IL-10 protein (100ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3226C
2.2 mL of human IL-10 antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3226D
120 μL of Streptavidin-HRP.
Assay Diluent
TBS3226E
12 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
