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Product Description
Description
The Fast Human IL-12 p70 ELISA is a solid phase ELISA designed to measure human IL-12 p70 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, with no need for 5-6 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human IL-12 p70 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-12 protein.
TYPICAL DATA
This standard curve (R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
SENSITIVITY
The minimum detectable dose (MOD) of human IL-12 p70 is typically 6.5 pg/ml.
The Intra-CV is < 10% and the Inter-CV is < 12%.
SPECIFICITY
This assay recognizes natural and recombinant human IL-12 P70.
No-cross reactivity: Human IL-12 p35; Human IL-12/IL-23 p40; Human IL-23; Mouse IL-12 p70.
SIMPLE PROCEDURES (Fig. 1)
SIMPLE STANDARD PREPARATION
STANDARD CURVE
KIT CONTENT AND STORAGE CONDITIONS
PART
PART#
DESCRIPTION
STORAGE OF OPENED/ RECONSTITUTED
Human IL-12 Microplate
TBS3204A
96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12 p70.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-12 Standard
TBS3204B
Recombinant human IL-12 protein (Lyophilized).
Reconstitute to 0.5 mL Assay Buffer.
Store at 2-8 °C for 6 months. After reconstituting in 0.5mL Assay Buffer, it can be stored at -20 ℃ for up to 1 month.
Detection A
TBS3204C
2.1 mL of human IL-12 antibody.
May be stored for up to 6 months at 2-8 °C.
Detection B
TBS3204D
0.25 mL of Streptavidin-HRP (1:50 Dilution).
Assay Diluent
TBS3204E
25 mL of a buffered protein base with preservatives.
The Fast Human IL-12 p70 ELISA is a solid phase ELISA designed to measure human IL-12 p70 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, with no need for 5-6 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human IL-12 p70 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-12 protein.
TYPICAL DATA
This standard curve (R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
SENSITIVITY
The minimum detectable dose (MOD) of human IL-12 p70 is typically 6.5 pg/ml.
The Intra-CV is < 10% and the Inter-CV is < 12%.
SPECIFICITY
This assay recognizes natural and recombinant human IL-12 P70.
No-cross reactivity: Human IL-12 p35; Human IL-12/IL-23 p40; Human IL-23; Mouse IL-12 p70.
SIMPLE PROCEDURES (Fig. 1)
SIMPLE STANDARD PREPARATION
STANDARD CURVE
KIT CONTENT AND STORAGE CONDITIONS
PART
PART#
DESCRIPTION
STORAGE OF OPENED/ RECONSTITUTED
Human IL-12 Microplate
TBS3204A
96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12 p70.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-12 Standard
TBS3204B
Recombinant human IL-12 protein (Lyophilized).
Reconstitute to 0.5 mL Assay Buffer.
Store at 2-8 °C for 6 months. After reconstituting in 0.5mL Assay Buffer, it can be stored at -20 ℃ for up to 1 month.
Detection A
TBS3204C
2.1 mL of human IL-12 antibody.
May be stored for up to 6 months at 2-8 °C.
Detection B
TBS3204D
0.25 mL of Streptavidin-HRP (1:50 Dilution).
Assay Diluent
TBS3204E
25 mL of a buffered protein base with preservatives.
