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AstorScientific | SKU: ELH-Adiponectin-1

Mouse Adiponectin ELISA Kit

Name: Mouse Adiponectin ELISA Kit
Brand: AstorScientific
SKU: ELH-Adiponectin-1
Price: 420.0 USD
Availability: InStock

$420.00

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Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Mouse
Accession Number	Q60994
Gene Id	11450
Gene Symbols	ADIPOQ\|ACRP30\|ACDC\|APM1
Protein Name / Synonyms	Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipocyte-specific protein AdipoQ)
Quantitative/Semi-Quantitative	Quantitative
Specificity	Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS-1, Angiopoietin-3, ANGPTL3, Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17 RB, IL-17B, IL-22, MIP-1 beta, MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM, Shh-N, TCK-1, TECK, TGFbeta 1, Trance, TREML1, TWEAK, VEGF-B, VEGFR2.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Research Area	Cardiovascular Disease
Sensitivity	49 pg/ml Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	49 pg/ml - 12,000 pg/ml
Recommended Dilution (Serum/Plasma)	20,000 fold
Estimated Lead Time	1-2 business days
Storage	≤-20°C

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

User Manual SDS

Citations

Allen, London, et al. "Effects of delta-tocotrienol on obesity-related adipocyte hypertrophy, inflammation and hepatic steatosis in high-fat-fed mice." The Journal of nutritional biochemistry 48 (2017): 128-137.

Species:Mouse Sample Type: Serum (adiponectin serum)

View Publication

Wang, Xin-An, et al. "CARD3 deficiency exacerbates diet-induced obesity, hepatosteatosis, and insulin resistance in male mice." Endocrinology 154.2 (2013): 685-697.

Species: Mouse Sample Type:

Serum (CARD3-knockout (KO) and wild-type (WT) mice fed a high-fat diet (HFD) to study obesity/insulin resistance)

View Publication

Wang, Xin-An, et al. "Interferon regulatory factor 7 deficiency prevents diet-induced obesity and insulin resistance." American Journal of Physiology-Endocrinology and Metabolism 305.4 (2013): E485-E495.

Species: Mouse Sample Type: Serum (C57BL/6 and IRF7 knockout mice - diet induced insulin resistance obesity model)

View Publication

Eo H., Jeon Yj., Lee M., et al. Brown Alga Ecklonia cava Polyphenol Extract Ameliorates Hepatic Lipogenesis, Oxidative Stress, and Inflammation by Activation of AMPK and SIRT1 in High-Fat Diet-Induced Obese Mice. J Agric Food Chem. 2014 Dec 22.

Species: Mouse Sample Type: Plasma (High-fat diet mice)

View Publication

Product Reviews

Size

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species

Mouse

Accession Number

Q60994

Gene Id

11450

Gene Symbols

ADIPOQ|ACRP30|ACDC|APM1

Protein Name / Synonyms

Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipocyte-specific protein AdipoQ)

Quantitative/Semi-Quantitative

Quantitative

Specificity

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS-1, Angiopoietin-3, ANGPTL3, Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17 RB, IL-17B, IL-22, MIP-1 beta, MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM, Shh-N, TCK-1, TECK, TGFbeta 1, Trance, TREML1, TWEAK, VEGF-B, VEGFR2.

Compatible Sample Types

Cell Culture Supernatants, Plasma, Serum

Solid Support

96-well Microplate

Method Of Detection

Colorimetric

Design Principle

Sandwich-based

Research Area

Cardiovascular Disease

Sensitivity

49 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.

Detection Range

49 pg/ml - 12,000 pg/ml

Recommended Dilution (Serum/Plasma)

20,000 fold

Estimated Lead Time

1-2 business days

Storage

≤-20°C

Mouse Adiponectin ELISA Kit

Care information

Delivery and Shipping

Payment & Security