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AstorScientific | SKU: ELM-Insulin-1

Mouse Insulin ELISA Kit

Name: Mouse Insulin ELISA Kit
Brand: AstorScientific
SKU: ELM-Insulin-1
Price: 420.0 USD
Availability: InStock

$420.00

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Product Description

Specifications

Size	1 Plate Kit, 5 Plate Kit
Species	Mouse
Accession Number	P01325
Gene Id	16333
Gene Symbols	INS1\|INS-1
Protein Name / Synonyms	Insulin-1 [Cleaved into: Insulin-1 B chain Insulin-1 A chain]
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA antibody pair detects mouse, human, rat, porcine and bovine Insulin.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	5 mIU/ml Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	5 uIU/ml - 400 uIU/ml
Recommended Dilution (Serum/Plasma)	2 fold
Estimated Lead Time	1-2 business days
Storage	≤-20°C

Target Background

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

User Manual SDS

Citations

Uday Chandrika, K., Tripathi, R., Kameshwari, Y. et al. Refunctionalization of Decellularized Organ Scaffold of Pancreas by Recellularization: Whole Organ Regeneration into Functional Pancreas. Tissue Eng Regen Med 18, 99-112 (2021). https://doi.org/10.1007/s13770-020-00296-y

Species:

Mouse

Sample Type:

Serum

View Publication

Liu Z, Ding Z, Lynch EC, McCauley N, Zhou Y, Zhang KK, Xie L. Pregestational diet transition to normal-fat diet avoids the deterioration of pancreatic ?-cell function in male offspring induced by maternal high-fat diet. J Nutr Biochem. 2020 Dec;86:108495. doi: 10.1016/j.jnutbio.2020.108495. Epub 2020 Sep 16. PMID: 32949717; PMCID: PMC7643480.

Species:

Mouse

Sample Type:

Plasma

View Publication

Arha, Deepti, et al. "Isoalantolactone derivative promotes glucose utilization in skeletal muscle cells and increases energy expenditure in db/db mice via activating AMPK-dependent signaling." Molecular and cellular endocrinology 460 (2018): 134-151.

Species:

Mouse

Sample Type:

Cell Lysate (rat skeletal muscle cells)

View Publication

Zhou Y, Liu Z, Lynch EC, He L, Cheng H, Liu L, Li Z, Li J, Lawless L, Zhang KK, Xie L. Osr1 regulates hepatic inflammation and cell survival in the progression of non-alcoholic fatty liver disease. Lab Invest. 2020 Oct 1. doi: 10.1038/s41374-020-00493-2. Epub ahead of print. PMID: 33005011.

Species:

Mouse

Sample Type:

Serum

View Publication

Abdel-Latif M. Diethylcarbamazine citrate ameliorates insulin resistance in high-fat diet-induced obese mice via modulation of adipose tissue inflammation. Int Immunopharmacol. 2015 Dec;29(2):607-12. doi: 10.1016/j.intimp.2015.09.021.

Species:

Mouse

Sample Type:

Serum (outbred swiss albino mice treated with Diethylcarbamazine citrate for insulin resistence)

View Publication

Mandaliya DK, Patel S, Seshadri S. The Combinatorial Effect of Acetate and Propionate on High-Fat Diet Induced Diabetic Inflammation or Metaflammation and T Cell Polarization. Inflammation. 2021 Feb;44(1):68-79. doi: 10.1007/s10753-020-01309-7. PMID: 32978698.

Species:

Mouse

Sample Type:

Serum

View Publication

Eo H., Jeon Yj., Lee M., et al. Brown Alga Ecklonia cava Polyphenol Extract Ameliorates Hepatic Lipogenesis, Oxidative Stress, and Inflammation by Activation of AMPK and SIRT1 in High-Fat Diet-Induced Obese Mice. J Agric Food Chem. 2014 Dec 22.

Species:

Mouse

Sample Type:

Plasma (High-fat diet mice)

View Publication

Species:

Mouse

Sample Type:

Plasma

View Publication

Othman, Azza I., et al. "Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats." Biomedicine & Pharmacotherapy 94 (2017): 362-373.

Species:

Rat

Sample Type:

Serum (diabetes)

View Publication

Majeed C., Al-Shammari A., Yausif E., et al. BM-MSCs Differentiated Insulin-Producing Cells Produce More Insulin in Presence of EGF than of FGF. Stem Cell Discovery 2015, 5, 33-39. http://dx.doi.org/10.4236/scd.2015.54004

Species:

Mouse

Sample Type:

Conditioned Media (BM-MSC cells)

View Publication

Product Reviews

Size

1 Plate Kit, 5 Plate Kit

Species

Mouse

Accession Number

P01325

Gene Id

16333

Gene Symbols

INS1|INS-1

Protein Name / Synonyms

Insulin-1 [Cleaved into: Insulin-1 B chain Insulin-1 A chain]

Quantitative/Semi-Quantitative

Quantitative

Specificity

This ELISA antibody pair detects mouse, human, rat, porcine and bovine Insulin.

Compatible Sample Types

Cell Culture Supernatants, Plasma, Serum

Solid Support

96-well Microplate

Method Of Detection

Colorimetric

Design Principle

Sandwich-based

Sensitivity

5 mIU/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.

Detection Range

5 uIU/ml - 400 uIU/ml

Recommended Dilution (Serum/Plasma)

2 fold

Estimated Lead Time

1-2 business days

Storage

≤-20°C

Mouse Insulin ELISA Kit

Care information

Delivery and Shipping

Payment & Security