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AstorScientific | SKU: ELM-Leptin-1

Mouse Leptin ELISA Kit

Name: Mouse Leptin ELISA Kit
Brand: AstorScientific
SKU: ELM-Leptin-1
Price: 336.0 USD
Availability: InStock

$336.00

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Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Mouse
Accession Number	P41160
Gene Id	16846
Gene Symbols	LEP\|OB
Protein Name / Synonyms	Leptin (Obesity factor)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF- 1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	4 pg/ml Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	4 pg/ml - 1000 pg/ml
Recommended Dilution (Serum/Plasma)	10 - 40 fold
Estimated Lead Time	1-2 business days
Storage	≤-20°C

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

User Manual SDS

Citations

Park J., Shim H., Na A., et al. Orexin A regulates plasma insulin and leptin levels in a time-dependent manner following a glucose load in mice. Diabetologia. 2015 Jul;58(7):1542-50. doi: 10.1007/s00125-015-3573-0.

Species:Mouse

Sample Type:

Plasma (Glucose Tolerance testing of high fat diet C57BL/6 mice)

View Publication

Li E., Shan H., Chen L., et al. OLFR734 Mediates Glucose Metabolism as a Receptor of Asprosin. Cell Metab . 2019 Aug 6;30(2):319-328.e8. doi: 10.1016/j.cmet.2019.05.022. Epub 2019 Jun 20.

Species:

Mouse

Sample Type:

Plasma (Asprosin in glucose metabolism mouse model)

View Publication

Naville, Danielle, et al. "Low-dose food contaminants trigger sex-specific, hepatic metabolic changes in the progeny of obese mice." The FASEB Journal 27.9 (2013): 3860-3870.

Species:

Mouse

Sample Type:

Plasma (obese mice)

View Publication

Wang, Xin-An, et al. "CARD3 deficiency exacerbates diet-induced obesity, hepatosteatosis, and insulin resistance in male mice." Endocrinology 154.2 (2013): 685-697.

Species:

Mouse

Sample Type:

Serum (CARD3-knockout (KO) and wild-type (WT) mice fed a high-fat diet (HFD) to study obesity/insulin resistance)

View Publication

Garg, S., et al. "Intervention of probiotic L. reuteri fermented milk as an adjuvant to combat protein energy malnourishment induced gut disturbances in albino mice." Journal of Functional Foods 36 (2017): 467-479.

Species:

Mouse

Sample Type:

Serum

View Publication

Wang, Xin-An, et al. "Interferon regulatory factor 7 deficiency prevents diet-induced obesity and insulin resistance." American Journal of Physiology-Endocrinology and Metabolism 305.4 (2013): E485-E495.

Species:

Mouse

Sample Type:

Serum (C57BL/6 and IRF7 knockout mice - diet induced insulin resistance obesity model)

View Publication

Wang X-A, Zhang R, Jiang D, et al. Interferon regulatory factor 9 protects against hepatic insulin resistance and steatosis in male mice. Hepatology. 2013 Aug;58(2):603-616.

Species:

Mouse

Sample Type:

Serum

View Publication

Bader, Manuela I., et al. "Comparative assessment of estrogenic responses with relevance to the metabolic syndrome and to menopausal symptoms in wild-type and aromatase-knockout mice." The Journal of steroid biochemistry and molecular biology 127.3 (2011): 428-434.

Species:

Mouse

Sample Type:

Serum

View Publication

Kishino E, Ito T, Fujita K, Kiuchi Y. A mixture of Salacia reticulata (Kotala himbutu) Aqueous Extract and Cyclodextrin Reduces the Accumulation of Visceral Fat Mass in Mice and Rats with High-Fat Diet-Induced Obesity. J Nutr. 2006;136:433-439.

Species:

Mouse

Sample Type:

Plasma

View Publication

Jiao, Ping, et al. "FFA?Induced Adipocyte Inflammation and Insulin Resistance: Involvement of ER Stress and IKK? Pathways." Obesity 19.3 (2011): 483-491.

Species:

Mouse

Sample Type:

Conditioned Media (3T3-L1 preadipocyte cell line)

View Publication

Product Reviews

Size

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species

Mouse

Accession Number

P41160

Gene Id

16846

Gene Symbols

LEP|OB

Protein Name / Synonyms

Leptin (Obesity factor)

Quantitative/Semi-Quantitative

Quantitative

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF- 1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

Compatible Sample Types

Cell Culture Supernatants, Plasma, Serum

Solid Support

96-well Microplate

Method Of Detection

Colorimetric

Design Principle

Sandwich-based

Sensitivity

4 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.

Detection Range

4 pg/ml - 1000 pg/ml

Recommended Dilution (Serum/Plasma)

10 - 40 fold

Estimated Lead Time

1-2 business days

Storage

≤-20°C

Mouse Leptin ELISA Kit

Care information

Delivery and Shipping

Payment & Security