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Product Description
Description
Melamine is a trimmer of cyanamide with the chemical formula C3H6N6, and the IUPAC name 1, 3, 5-triazine-2, 4, 6-triamine. It is widely used in manufacturing plastics, dyes, fertilizers, and textiles. Like cyanamide, it is 66% nitrogen by mass, which leads to the practice in various countries of adding “melamine scrap” to animal feed, pet food, or milk in order to give the appearance of increased protein content. This practice can potentially contaminate animal products intended for human consumption like dairy products. Animal and human consumption of melamine could lead to kidney stones and renal failure, causing severe consequences including possible death.
Tribioscience’s Melamine Fast ELISA kit allows for fast (entire procedure in 30 minutes), simple (direct analysis for dairy products; only homogenization and water extraction are needed for animal feed or pet food), sensitive and reliable detection of melamine contamination in animal feed, pet food and dairy products such as milk, milk powder and yogurt. If necessary, samples requiring regulatory action can be confirmed by HPLC, GC/MS, or other conventional methods.
Simple Procedures
Standard Preparation
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Pre coated Microplate
TBS21104A
96 well microplate (12 strips of 8 wells) coated with an antibody specific for melamine.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Melamine Standard
TBS21104B
60 µL of melamine (8.1 µg/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS21104C
200 µL of HRP- melamine conjugate (100x)
May be stored for up to 3 months at 2-8 °C.
Assay Diluent
TBS21104D
25 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
10 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 1 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit is sufficient for one 96-well plate ELISA.
Melamine is a trimmer of cyanamide with the chemical formula C3H6N6, and the IUPAC name 1, 3, 5-triazine-2, 4, 6-triamine. It is widely used in manufacturing plastics, dyes, fertilizers, and textiles. Like cyanamide, it is 66% nitrogen by mass, which leads to the practice in various countries of adding “melamine scrap” to animal feed, pet food, or milk in order to give the appearance of increased protein content. This practice can potentially contaminate animal products intended for human consumption like dairy products. Animal and human consumption of melamine could lead to kidney stones and renal failure, causing severe consequences including possible death.
Tribioscience’s Melamine Fast ELISA kit allows for fast (entire procedure in 30 minutes), simple (direct analysis for dairy products; only homogenization and water extraction are needed for animal feed or pet food), sensitive and reliable detection of melamine contamination in animal feed, pet food and dairy products such as milk, milk powder and yogurt. If necessary, samples requiring regulatory action can be confirmed by HPLC, GC/MS, or other conventional methods.
Simple Procedures
Standard Preparation
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Pre coated Microplate
TBS21104A
96 well microplate (12 strips of 8 wells) coated with an antibody specific for melamine.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Melamine Standard
TBS21104B
60 µL of melamine (8.1 µg/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS21104C
200 µL of HRP- melamine conjugate (100x)
May be stored for up to 3 months at 2-8 °C.
Assay Diluent
TBS21104D
25 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
10 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 1 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit is sufficient for one 96-well plate ELISA.