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Product Description
Description
The Fast Mouse G-CSF ELISA is a solid phase ELISA designed to measure mouse G-CSF levels in cell culture supernates, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of mouse G-CSF samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF protein.
Kit Contents and Storage Conditions
Reagents
Quantity
Mouse G-CSF Capture
96 well microplate (12 strips of 8 wells) coated with a capture antibody against mouse G-CSF.
Mouse G-CSF Standard
20 µl of Recombinant mouse G-CSF protein (100ng/mL).
Detection A
2.1 mL of mouse G-CSF antibody-Biotin.
Detection B
240 µl of Streptavidin-HRP (50x).
Assay Diluent
20 ml of a buffered protein base with preservatives.
10x WashBuffer
12ml of concentrated solution (10x).
TMB Substrate
12 ml of ultra-sensitive TMB substrate.
Stop Solution
6ml of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
Fig. 1 Simple Assays Procedures
Fig. 2 Diagram for Mouse G-CSF standard preparation
The Fast Mouse G-CSF ELISA is a solid phase ELISA designed to measure mouse G-CSF levels in cell culture supernates, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of mouse G-CSF samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF protein.
Kit Contents and Storage Conditions
Reagents
Quantity
Mouse G-CSF Capture
96 well microplate (12 strips of 8 wells) coated with a capture antibody against mouse G-CSF.
Mouse G-CSF Standard
20 µl of Recombinant mouse G-CSF protein (100ng/mL).
Detection A
2.1 mL of mouse G-CSF antibody-Biotin.
Detection B
240 µl of Streptavidin-HRP (50x).
Assay Diluent
20 ml of a buffered protein base with preservatives.
10x WashBuffer
12ml of concentrated solution (10x).
TMB Substrate
12 ml of ultra-sensitive TMB substrate.
Stop Solution
6ml of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
Fig. 1 Simple Assays Procedures
Fig. 2 Diagram for Mouse G-CSF standard preparation