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Product Description
Description
The Fast Mouse IFN-γ ELISA is a solid phase ELISA designed to measure mouse IFN-γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of mouse IFN-γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-γ protein.
Kit Contents
Reagents
Description
Mouse IFN- γ Microplate
96 well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for mouse IFN- γ.
Mouse IFN- γ Standard
30 µl of Recombinant mouse IFN- γ protein (100ng/mL).
Detection A
2.1 ml of mouse IFN- γ antibody.
Detection B
12 ml of Streptavidin-HRP
Assay Diluent
15 ml of a buffered protein base with preservatives.
10x WashBuffer
12ml of concentrated solution(10x).
TMB Substrate
12 ml of ultra-sensitive TMB substrate.
Stop Solution
6ml of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Fig.1 Simple Assay Procedures
Fig. 2 Diagram for Mouse IFN-γ standard preparation
The Fast Mouse IFN-γ ELISA is a solid phase ELISA designed to measure mouse IFN-γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of mouse IFN-γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-γ protein.
Kit Contents
Reagents
Description
Mouse IFN- γ Microplate
96 well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for mouse IFN- γ.
Mouse IFN- γ Standard
30 µl of Recombinant mouse IFN- γ protein (100ng/mL).
Detection A
2.1 ml of mouse IFN- γ antibody.
Detection B
12 ml of Streptavidin-HRP
Assay Diluent
15 ml of a buffered protein base with preservatives.
10x WashBuffer
12ml of concentrated solution(10x).
TMB Substrate
12 ml of ultra-sensitive TMB substrate.
Stop Solution
6ml of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Fig.1 Simple Assay Procedures
Fig. 2 Diagram for Mouse IFN-γ standard preparation