Tribo™ Mouse TNF-α ELISA Kit
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Tribo™ Mouse TNF-α ELISA Kit
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Product Description
Description
Tribioscience’s Fast Mouse TNF-α ELISA is designed to quantitatively detect mouse TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 31 to 2000 pg/mL. The levels of mouse TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse TNF-α protein.
Typical Data
This standard curve (R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of mouse TNF-α is typically 10 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant mouse TNF-α.
No cross-reactivity: human TNF- α, β; human TNF RI, human TNF RII; Porcine TNF-α; Rat TNF-α; Mouse TNF RI, Mouse TNF RII.
Simple Procedures (Fig. 1)
Simple Standard Preparation (Fig.2)
Accurate Standard Curve (Fig.3 )
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Mouse TNF-α Microplate | TBS3050A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for mouse TNF-α. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Mouse TNF-α Standard | TBS3050B | 20 µL of Recombinant mouse TNF-α protein (100 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3050C | 2.2 mL of Biotin-mouse TNF-α antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3050D | 200 µL of Streptavidin-HRP. | |
| Assay Diluent | TBS3050E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
